The objective of this study was to understand the distribution pattern of microsatellite (SSR) and to develop EST-SSR primers to provide effective identification markers for the evaluation of germplasm resources and assisted breeding of Huaren apricot. In this study, the number, frequency and distribution of SSR loci in young apricot fruit were analyzed by bioinformatics. The SSR primers were screened and developed using transcriptome data. Polymorphic loci in 29 clones of Huaren apricot were detect and identify using the developed primers. The results showed that the frequency of EST-SSR was 19.21% and the repetitive number of repeat units was between 5 and 24 times. The dominant repeat motifs were single nucleotide, dinucleotide and trinucleoside Acid, accounting for 19.81%, 46.47%, 32.49% of the total SSR respectively. Among the 139 pairs of primers tested, 39 pairs of primers amplified the target sequence, of which 24 pairs of primers could detect polymorphic sites, accounting for 17.27% of the total number of primers tested. A total of 170 alleles were detected in 29 Huaren apricot clones using 24 pairs of primers. The PIC ranged from 0.33 to 0.87 with an average of 0.64, of which, high polymorphism primer 19 pairs, accounting for 79.2% of the proportion of polymorphic primers. In this study, SSR information of Huaren apricot transcriptome was analyzed and 19 pairs of high polymorphic SSR primers and 5 pairs of moderate polymorphic primers were developed, which provides the foundation for evaluating the germplasm resources of Huaren apricot and molecular markers Breeding.