A RING-type E3 ubiquitin ligase SDIR1 (SALT-AND DROUGHT INDUCED REALLY INTREASTING NEW GENE FINGER1), which plays a pivotal role in plants signaling pathway. TaSDIR1-4D was isolated from genome D of wheat (Triticum aestivum L.). The full length of TaSDIR1-4D was 4,070 bp, including a 1443 bp upstream fragment of the coding region, 2352 bp coding region, 275 bp 3' UTR.The gene cDNA sequence length of the coding region was 849 bp, which encoded 282 amino acids, including two transmembrane domains and a relatively conserved RING finger domain. TaSDIR1-D was located on the chromosome 4D by using wheat wild relatives and a set of nullisomic-tetrasomic lines of Chinese Spring. TaSDIR1-D showed the highest expression level in flag leaves during heading stage. TaSDIR1-D was up-regulated by NaCl, ABA, PEG and 4℃ treatments, which suggested that TaSDIR1-D may be involved in plant abiotic stress signaling pathway. By detecting the polymorphism of TaSDIR1-D gene sequence in 32 hexaploid wheat accessions, two SNPs were detected in the full-length sequence. One was identified in the promoter region, another in the fourth exon (G/A), which led to amino acid Arg (CGC) change into His (CAC). In a natural population consisted of 262 accessions, two haplotypes of TaSDIR1-D were detected by SNP-583, a SNP marker developed from the SNP (T/C) at -583 bp, which were significantly or very significantly correlated with 1000-grain weight, length of penultimate node and spike length. Hap-4A-2 is considered as a potential superior haplotype for increasing 1000-grain weight. The results provide a theoretical basis and genetic resources for wheat molecular breeding.