In order to understand the characteristics of fruit brush in grape cultivars, this study analyzed the fruit brush related traits in 126 grape cultivars and the fruit hole browning in 74 varieties at the ripening stage. The results show that there are some differences in the characteristics of fruit brush between varieties. For instance, the fruit brush is either complete or incomplete, and the condition of sticking pulp is different when the brush is pulled out. Vitis vinifera L. fruit brush and pulp adhesion is higher than V. vinifera × V. labrusca L. There was a significant positive correlation between the pull resistance of Vitis vinifera L. grape fruit brush and the length of fruit brush and the thickness of fruit brush; however, there was no correlation between the pull resistance of V. vinifera×V. labrusca L. grape fruit brush and the length of fruit brush, but there is a significant positive correlation between brush thicknesses and the pull resistance.In addition, the pull resistance of Vitis vinifera L. species was significantly greater than that of V. vinifera×V. labrusca L. The browning speed and browning degree of the flesh are different among the varieties, and the higher the maturity, the lower the browning degree is. The browning speeds of different varieties of fruit holes are inconsistent, and the different sizes of fruit holes cause different degrees of damage to the fruit, which affects the storage stability of the postharvest fruit to a certain extent. By deciphering the diversity of fruit brush, this work provided a considerable theoretical basis in evaluation of grape germplasm resources and in breeding of elite varieties.