Enolase ENO2, which is widely found in animals and plants, is a key glycolytic enzyme. However, latest studies have shown that it has a variety of functions in addition to participating in the glycolytic pathway. In plants, ENO2 can affect the growth and reproductive development of plants, response to abiotic stresses and has the function of transcriptional regulation. Especially, mRNA expressed by ENO2 can alternatively be translated into 2 proteins, ENO2 and MBP-1. Therefore, we summarized the various functions of plant ENO2 and the related research progress. Moreover, we also discuss the current research trends about plant gene ENO2 in order to guide the further research in related fields.