HbWRKYs is a family of transcription factor genes in Hevea brasiliensis, which is involved in the physiological and biochemical processes such as gum production, senescence and stress resistance of Hevea brasiliensis. Phytohormones (such as ethephon, jasmonic acid, and salicin acid, etc. ),pathogen infestation and mechanical injury can all induce the expression of HbWRKYs . At present, most members of this gene family have been cloned and analyzed, but the exact location of these genes on the chromosome has not yet been determined. In this paper, useing Hevea brasiliensis ‘Reyan 7-33-97’ as the material and the technique of FISH and in situ PCR were used to identify the 10 members of HbWRKYs (HbWRKY1, HbWRKY2, HbWRKY3, HbWRKY4, HbWRKY5, HbWRKY7, HbWRKY9, HbWRKY11, HbWRKY27 and HbWRKY75). The results showed that the HbWRKY1, HbWRKY4 and HbWRKY5 genes were located on chromosome 9 at the same time, in which HbWRKY1 and HbWRKY4 were located on the short arm of chromosome 9. The average distances of their signal sites from centromeres were 57.38 and 4.57, respectively. HbWRKY5 was located on the long arm of chromosome 9, the average distance from the centromeric signal site is 31.67; HbWRKY2, HbWRKY3, HbWRKY7, HbWRKY9, HbWRKY11 and HbWRKY75 genes were mapped on the short arms of chromosomes 8, 3, 4, 10, 2, and 7,and the mean distances from the centromeres of signal sites were respectively 6.00, 12.30, 4.40, 45.21, 5.74 and 26.11 ;The HbWRKY27 gene is located on the long arm of chromosome 6, and the mean distance from the centromeric signal site is 38.88. This article also discusses the positional relationship between these genes and other mapped genes.