Winter freezing seriously threatens the yield stability of common wheat in the north area of China. Test for freezing resistance under the field condition largely depended on the environmental climate. By taking use of artificial freezing, we investigated the relative conductivity and freezing survival rate of the plants under different freezing treatments in 209 wheat varieties, with a purpose of obtaining excellent variety resources for cold resistance breeding of wheat. Furthermore, we analyzed the correlation of relative conductivity, freezing survival rate and lethal temperature with field frost damage grade. The results showed a significant negative correlation between the freezing survival rate and freezing injury level after treatment for 3h at -14°C (correlation coefficient = -0.427). By cold treatment under different temperatures, we found the optimal temperature condition that could be used to identify the cold resistance at seedling stage. As a result, several cold resistance varieties such as Henong 05 (9) 7-11-4-15, Jimai 585, Le 639, Shimai 15, Bao 5108, Shixin 828, Zhongmai 1 and Henong 7069 were obtained.