In this study, ninety-six rice core germplasm resources and these 47 hybrid progeny in Ningxia province were tested for rice blast resistance in the fields, by using artificial inoculation and natural infection with physiological races of rice blast pathogen. Moreover, eight functional markers, which associated to the respective rice blast resistance genes, were used for marker-assisted detection of the resistance genes in mentioned materials as well as additional 28 different Genotypes. As observed, the resistance of genotypes containing Pita, Pib, Pikm, Pikh, P9 and Pi5 represented significantly high level of resistance than that of other genotypes. The Pita gene, pyramiding with either of Pikm, Pikh, Pi2 and Pi5 genes, showed increased resistance to rice blast, and notably, stacking of Pita and Pikh genes showed highest resistance. However, Pib and Pita+Pikh in a genotype showed reduced rice blast resistance. Thus, this work provides an important reference in breeding for new rice varieties with broad-spectrum resistance, by pyramiding of rice blast resistance genes in Ningxia region.