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Home > Archive>Volume 20, Issue 2, 2019 >370-376. DOI:10.13430/j.cnki.jpgr.20180810001
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Construction of EMS-treated Mutant Library and Mutant Phenotypic Analysis in Broomcorn Millet
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1College of Agricultural, Shanxi Agricultural University, Taigu 030801;2Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801;3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030001;4 Shanxi Key Laboratory of Resources and Genetic Improvement of Minor Crops, Taiyuan 030001

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This work is supported by the key discipline foundation of Shanxi province (201603D221003-6), National Natural Science Foundation of China (31601369, 31371693, 31471556), and the scientific and technological innovation breeding programs of Shanxi Agriculture University (20132-23).

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    Abstract:

    Broomcorn millet (Panicum miliaceum L.) is one of the minor crops in China, which represented resistant and tolerant against drought, lower-fertilizer and abiotic stresses as well as short life-cycle. Within this study, by generating an EMS-triggered mutant population by using broomcorn millet variety Yi-Xuan-Da-Hong-Mi, we analyzed the mutation frequency and phenotypic variation of 2333 M2 plants. Out of that, a total of 103 plants with visible phenotype differing with the parental line were identified, yielding 4.4% of mutation rate. These mutants showed modifications on any of leaf color, leaf shape, plant height, mature period, fertility, spike shape, seed-coat color and so on. Thus, these mutants would become a genetic resource being useful in deciphering the gene function and breeding for new varieties in Broomcorn millet.

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History
  • Received:August 10,2018
  • Revised:November 15,2018
  • Adopted:December 11,2018
  • Online: March 19,2019
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