Lobed-leaf is widely known as an ideal morphological marker in rapeseed breeding. Genetic variation of lobed-leaf plays an important role in enriching the leaf shape diversity and improving genetic breeding of rapeseed. In this study, fifty plants with normal leaf and 50 plants with lobed-leaf were selected from the F2 population respectively, which was derived from a cross of the normal leaf line Zhongshuang No.11 and the leaf-lobed new line Y176. Two bulked DNA pools were generated, which were subjected for SLAF-seq-BSA (specific-locus amplified fragment sequencing and bulked segregant analysis). A total of 177,941 labels were obtained and 150,168 SNPs were identified, including 128,200 SNPs that were polymorphic between both parents. A physical interval expanding ~2.83 Mb on chrA10 (14,528,149~17,353,693 bp) was tightly associated with lobed-leaf trait. Within that, 666 candidate genes was found. Since the genetic confidence interval of this locus differed to the physical positions of previously reported genes, there might be a new gene controlling leaf margin cleavage. Furthermore, 666, 517, 587, 151 and 258 genes in this region were annotated by NR, Swiss-Prot, GO, KEGG and COG databases, respectively. Thus, this information might be helpful in future map-based gene isolation of lobed-leaf in Brassica napus L.