The seed production potential of male sterile lines is the main factor affecting hybrid seed productivity. The lack of early selection method always leads to low hybrid seed yield and unsuitable for application. A collection of radish landraces with high quality have been developed in China over decades, but nowadays these germplasms showed characterization declining, cultivation area reducing and scattering. Hybrid breeding is an effective way to improve the agronomic quality, maintain the cultivation area and expand the planting region of the landraces. By hybridization using the Ogura cytoplasm with 26 radish landraces, 16 introgression lines were absolute or near absolute male sterile. A osmia pollination and nylon mesh isolation method were used to test the seed production potential of the male sterile and maintainer lines of these 16 landraces. The two accessions, Lian-si and May-Red, showed high silique yield in both male sterile and maintainer plants, with the potential of developing high-seed-yielding male sterile lines. The Red-lanterns, Big-green-skin and Beautiful-in-heart contain high silique yield plants in male sterile lines while all maintainer plants showed low yield. Improving the seed productivity of maintainer lines are needed for these three landraces. The Little-red-robe summer radish and Fu-gou radish produced medium quantity of siliques from both male sterile and maintainer plants, have application potential if substantial selection can improve the seed yield. The Red summer radish, Green crisp and three other landraces were proved not suitable to produce hybrid seeds use Ogura-CMS due to low yield. The Dog-Canister, Jiang-xian-white and Ivory were identified as self incompatibility materials. This study revealed that the seed yield are differing significantly among varieties and single plants, indicating a necessary of selecting the high-yielding plant descendants with generations. Thus, this worked reported a simple and efficient method for screening the seed production potential, which finally resulted in generation of seven Ogura-CMS radishes with high seed yielding potential .