The species of Chrysosplenium has significant pharmacological effects due to the flavonols component. In this study, a detailed field scientific expedition of Chrysosplenium germplasm resources in five provinces of China (Tibet, Yunnan, Sichuan, Qinghai, and Gansu) was performed. 19 species (including two varieties) and 145 specimens of Chrysosplenium were collected. In addition, two newly recorded species of Chrysosplenium (C. biondianum & C. qinlingense) were found in Sichuan province, and both were extensively found in China. Three new records were observed in Gansu province, including C. pilosum, C. lanuginosum and C. henryi. These specimens were preserved in the Herbarium of South-Central University for Nationalities (HSN). Thus, this work provided additional wild resources of Chrysosplenium in Tibetan-inhabited areas, which might be helpful in further classification of this genus.