2025-5-6- 16
Home > Archive>Volume 20, Issue 4, 2019 >1020-1025. DOI:10.13430/j.cnki.jpgr.20181015002
PDF HTML XML Export Cite reminder
Stable Transformation of Haloxylon ammodendron HaNAC1 Gene to Improve Drought Resistance of Potato
Author:
Affiliation:

Agricultural Bio-Technology Centre, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002

Fund Project:

Nation Science Foundation Project(31560053), Ningxia Agriculture Breeding Special Project(2014NYYZ01), Ningxia Academy of Agriculture and Forestry's 13th Five-Year Major Scientific Research Project (YES-16-0102), Ningxia Science Foundation Project (NZ16123, NZ16122, NZ17124,NZ14210), Ningxia Academy of Agriculture and Forestry Science and Technology Innovation Pilot Fund Project (NKYJ-17-03, NKYJ-17-07, NKYJ-17-19,DWHZC-2017002)

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    NAC transcription factors are involved in plant abiotic stress response and play important roles for improving plant drought and salt tolerance. Taking advantage of established agrobacterium-mediated transformation platform using the potato cultivar "Atlantic", we generated the transgenic potatoes by expressing Haloxylon ammodendron HaNAC1, and conducted tests for drought resistance with PEG-6000 simulated drought treatment. By analyzing the contents of endogenous hormones, these results showed significant increased content of auxin (IAA, IBA), cytokinin (IP, cZ), jasmonic acid (MEJA, JA-ILE), salicylic acid (MESA), gibberellin (GA3) in three transgenic potato lines if compare to the receptor line (p<0.01, p<0.05). The content of abscisic acid (ABA) was significantly lower than the receptor line. By analyzing the transcriptional level using quantitative PCR analysis, several stress-tolerant genes including NCED1, ERD11, RD29A, DREB2A, LEA3 and KIN1 showed the modified expression in HaNAC1 overexpressing plants to the receptors (p<0.01, p<0.05). Taken together, our results suggested that increased drought resistance by expressing HaNAC1 is associated with the different expression of stress-related genes and hormone content, thus providing a theoretical basis for further molecular mechanism analysis of HaNAC1 response to drought stress.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 15,2018
  • Revised:April 11,2019
  • Adopted:January 15,2019
  • Online: July 16,2019
Article QR Code
You are the 634911th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.