In order to determine the resistance difference of sorghum germplasm and the pathogenicity difference of Sarocladium strictum from sorghum and maize, a total of 110 sorghum germplasm accessions were subjected for evaluation for the resistance to Black Bundle Disease under artificial inoculation conditions from 2016 to 2017. With this collection, 13 lines (11.8%) were scored as immune (IM), 17 lines (15.5%) were highly resistant (HR), 14 lines (12.7%) were resistant (R) and 14 lines (12.7%) were moderately resistant (MR), 17 lines (15.5%) were susceptible (S), 35 lines (31.8%) were highly susceptible (HS) to GHS-1. Twenty-one lines (19.1%) were immune (IM), 11 lines (10.0%) were highly resistance (HR), 15 lines (13.6%) were resistance (R), 12 lines (10.9%) were moderately resistance (MR), 21 lines (19.1%) were susceptibility (S) and 30 lines (27.3%) with highly susceptibility (HS) to YHS-1. These results showed that a proportion of restorer lines widely used in sorghum breeding were resistant to Black Bundle Disease, and the pathogenicity of Black Bundle Disease pathogens isolated from sorghum and maize was different.