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Home > Archive>Volume 20, Issue 4, 2019 >845-853. DOI:10.13430/j.cnki.jpgr.20181029001
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Construction of DNA Profile Database of Wheat Reference Varieties and Its Application in Wheat DUS Test
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Crop Research Institute, Shandong Academy of Agricultural Sciences

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Public welfare industry (Agriculture) research projects

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    Abstract:

    Based on 2,256 common wheat reference varieties for DUS test in China, a DNA profile database was established to assist in distinctness test. We first analyzed the DNA profiles of 96 reference varieties using 81 fluorescently labeled SSR markers. A total of 731 alleles were detected with the number of alleles varying between 3 and 16 and the PIC values from 0.24 to 0.82. A DNA profile database consiting of 2256 wheat reference varieties was constructed using 42 fluorescently labeled SSR markers. A total of 148,493 datapoint were included with a missing rate of less than 5%. The genetic and genotypic datasets of 176 wheat varieties were collected and analyzed. The genetic similarities between the non-distinct candidate varieties and their most similar vairieties were above 90%. The candidate varieties with a genetic similarity of over 80% are assumed to be similar varieties, which are suggested to be further investigated for phenotypic variations in the field.

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History
  • Received:October 29,2018
  • Revised:March 01,2019
  • Adopted:March 14,2019
  • Online: July 16,2019
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