In order to identify the resistance and leaf rust resistance genes of 12 wheat cultivars from Hebei province, we conducted tests for leaf rust resistance using 20 Puccinia triticina pathotypes at the seedling stage of 12 wheat cultivars (lines) as well as 37 cultivars (lines), which are the resistant donors with known leaf rust resistance genes. Meanwhile, marker-assisted identification using 11 molecular markers closely linked were performed. In order to identify the adult plant leaf rust resistance, , the diseases severity of the tested materials was investigated by field trails in 2014-2015 and 2015-2016 cropping seasons at Zhoukou, Henan Province and Baoding, Hebei Province. Four leaf rust resistance genes including Lr1, Lr26, LrB and Lr46, were detected in 12 wheat cultivars (lines), and eight cultivars (lines) may carry Lr26. These cultivars Shixin 618, Gaoyou 2018 and Ji 5265 may carry Lr1, LrB, Lr46 respectively. As indicated by field identification in two field growing seasons, Shixin 733, Gaoyou 2018 and Shiyou 17 showed slow delayed establishment of leaf rust disease symptom rusting, and they can be used as the resistance donors for breeding.