The Distribution of Low Amylose Content Allele Wx-mp in japonica Rice (Oryza sativa L. )
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1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement / Jiangsu High Quality Rice R & D Center, Nanjing 210014;2Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225009

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the National Key Research and Development Program (2017YFD0100403), the Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)2022), and the Jiangsu Province Key Research and Development Program (Modern Agriculture, BE2017368

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    Abstract:

    The rice varities harboring Wx-mp genotype generally results in 10% of amylose, which brings elite eating quality (e.g soft and elastic) favorited by consumers in Yangtze River Region, China. In order to identify Wx-mp carrying rice germplasm in future breeding, a functional marker associating to Wx-mp has been developed. By tests in 242 japonica rice materials collected from southern China, twenty-six germplasms were found carrying Wx-mp gene. Out of that, 25 varieties or breeding materials were collected from Jiangsu, the others from Shanghai. By validation using Sanger sequencing, this newly-developed marker proved 100% accuracy within 26 samples. Furthermore, average content of amylose was 9.2%, with a range of 3.3%. The rice cultivar Nanjing 505 with the lowest amylose content was 7.3, while cv. Nanjing 46 showed a highest amylose content of 10.6%. Thus, by deployment of the new functional marker, this study unlocked the distribution of Wx-mp carrying cultivars that were was mainly found in Jiangsu and shanghai Province.

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History
  • Received:November 21,2018
  • Revised:April 30,2019
  • Adopted:January 15,2019
  • Online: July 16,2019
  • Published:
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