SQUAMOSA PROMOTER BINDING PROTEIN-box (SBP-box) genes, that belong to plant-specific transcription factors, are involved in many important aspects including leaf?development, embryogenesis, vegetative and?reproductive?phase transitions. This family has important practical application in crop genetic improvement. In this study, we isolated a sorghum SBP-box gene named SbSBP5, and conducted the sequence analysis and the transcriptional pattern under different biotic stresses. SbSBP5 coded for a polypeptide of amino acids that contained a typical SBP domain. By analyzing the promoter sequence, several stress-related cis-elements including MBRE, ARE and MBS were detected. Twelve and 11-fold up-regulation of SbSBP5 was detected by PEG-directed drought treatment or applying exogenous abscisic acid. In transgenic Arabidopsis that expressed SbSBP5 promoter plus a GUS reporter, three and four fold of GUS activity were detected under drought or exogenous abscisic acid treatments, respectively. Thus, these results provided preliminary outcome of SbSBP5 responding to drought and exogenous abscisic acid, which might provide a basis for further unlocking its molecular function.