Five blast resistance (R) genes, Pib, Pita, Pi5, Pi25 and Pi54, which showed relative broad-spectrum resistance to Chinese Magnaporthe oryzae isolates, were surveyed in the mini-core collection of Chinese rice germplasm by using their functional markers. As a result, 124 accessions of germplasm were found to harbor 1-4 target genes, respectively. Among them, Huangsiguizhan contained four R genes including Pib, Pita, Pi5 and Pi54;Nanxiongzaoyouzhan and Wuzuihonggu each harbored three R genes Pita/Pi25/Pi54 and Pita/Pi5/Pi54, respectively；35 accessions including Yelicanghua and Liaojing 287, etc. each harbored two R genes with genotypic constitutions of Pi5 and Pi54, Pita and Pi54, Pib and Pi54, Pib and Pita, Pi25 and Pi54, Pib and Pi5, Pita and Pi5, Pita and Pi25, Pi5 and Pi25, respectively. 86 accessions including Funingzipijingzi and Longhuamaohulou, etc. each harbored a single target gene. These results will provide references for grasping blast R genotypes of themini-core collection of rice germplasm and improving cultivar resistance using the elite accessions of germplasm.