Shiwandashan Mountain in Guangxi is an important area with abundant biodiversity. Surveying the diversity of Crop Germplasm Resources in this area is of great significance in protection and exploration of local crop germplasm/gene resources. By use of 181 germplasm resources, which were collected in a frame of the Third National Survey and Collection Action on Crop, the clarification of species, geographic distribution and biodiversity were investigated. These germplasm accessions were clarified in 48 species, 39 genera and 22 families. That included 78 feeding grain crops, 53 industrial crops, 39 vegetables and 11 fruit trees, including 146 local landraces and 35 wild accessions. Due to the unbalanced industrial development and adjustment of modern crop planting structure, the distribution and biodiversity of crop germplasm resource in Shiwandashan Mountain are constantly decreasing and many excellent germplasm resources are rarely found. These findings suggested a strategy on ectopic conservation and original habitat protection, which might promote the sustainable utilization of local excellent germplasm resources valuable for Rural Revitalization and economic construction.