In flowering plants, 14-3-3 protein plays an important regulatory role in plant growth and development. In this experiment, a 14-3-3 protein family gene Lb14-3-3c was cloned from the anther of Ningqi No.1 by reverse transcription PCR (RT-PCR). The expression characteristics of Lb14-3-3c gene in different stages of anther development were analyzed by real-time PCR. The plant over-expression vector pCambia1305.1-35s-Lb14-3-3c(+) of Lb14-3-3c gene and plant inhibition were constructed. The vectors were transformed into Agrobacterium GV3101 by freeze-thaw method, and then transformed into Astragalus membranaceus by Agrobacterium-mediated transformation. The expression of Lb14-3-3c gene in positive transformed seedlings and leaf starch content were analyzed. Bioinformatics analysis showed that the complete open reading frame (ORF) of Lb14-3-3c gene is 777bp long and has a conserved domain of 14-3-3 protein family. The molecular weight of the protein is 64.14 kD and the isoelectric point is 4.95. The grade structure is mainly α-helix. The amino acid sequence has a similarity to tobacco 14-3-3c-1 of 90.04%. The protein encoded by Lb14-3-3c gene is on the same branch of tomato as the phylogenetic tree, and has the closest relationship. Real-time PCR analysis showed that the gene was expressed in all organs of the sputum, and the expression was highest in the stamens. It is expressed in all stages of anther development and has the highest expression during microspore mother cell. The exogenous gene Lb14-3-3c was successfully loaded into the plant expression vector containing the strong promoter CaMV35s, and the plant overexpression vector of the gene was transformed into potato. Five transgenic plants were identified by phenotypic observation and PCR positive identification. The growth rate of transgenic plants was better than that of wild-type plants. The content of wild-type and transgenic starch in seedling stage was similar. The starch content of transgenic potato in potato and mature stage was higher than that in wild type. This study provides a reference for the further study of Lb14-3-3c gene regulation of starch supply during the development of anthers, and provides a demonstration of the function of Lb14-3-3c gene during plant development and molecular genetic improvement.