Under salt stress from the soil, the economic yield of wheat will decrease due to reduced uptake of water and nutrients. The enhancement of root tolerance to salt will be one of the important ways in wheat breeding for salt-tolerance. It will significantly raise root salt-tolerance by driving salt-tolerance genes preferentially expressed in roots, greatly induced by salt stress, while isolation and characterization of the promoter with dual functions will be the basis of precise control of salt-tolerance genes. Hence, in this study, we screened and determined 425 probes, and selected 2 candidate probes from them for further promoter validation. The roots of one-week-old seedlings of wheat variety ‘Chinese Spring’ were placed in 200 mM NaCl solution, and sampled at 0 h, 0.5 h, 1 h, 2 h, 4 h and 8 h for analysis of gene expression patterns. The results showed that the gene corresponding to Ta.5463.1.A1_at probe was fitting better into the expected bioinformatic results, which is preferentially expressed in roots and up-regulated by the induction of salt stress. To further verify the functions of its promoter, the promoter region was cloned and ligated into the promoter verification vector, and transgenic Arabidopsis plants were obtained. The results of salt induction and GUS staining showed that the promoter could regulate the expression of GUS reporter gene under salt treatment significantly, and the reporter gene was mainly expressed in roots. Our study successfully isolated specific promoter for salt tolerance, and provided an excellent resource for molecular breeding in wheat stress resistance.