With the increase of crop germplasm resources, the increasingly frequent exchange of germplasm resources and the gradual deepening of the utilization of germplasm resources, the construction of crop germplasm resources database is very important for the application research and basic research of crops. Shanghai Agricultural Biological Gene Center has developed a set of Shanghai crop germplasm resources database，based on the information of nearly 210000 crop germplasm preserved by the Center. This paper mainly describes the purpose and principle of the database design and construction, architecture of the database, running environment and main function modules..The database of Shanghai Crop Germplasm Resources inadopts B/S structure, and the platform system realizes the collection, storage, query, statistical analysis and other functions of crop germplasm resources data using multi-layer logical structure. External users can access the platform system through browser, browse and query germplasm resources information. The internal administrator can use the limited function of the platform and manage the whole platform data, when the authentication is successful. Registered users can also submit the introduction application through the gene resource database, and administrators can carry out network management for the whole process of external supply. This can improve work efficiency and increase the transparency of workflow.