Distant hybridization is an important approach in crop genetic breeding and germplasm creation. In this study，the F1 hybrids were produced by interspecific hybridization between B. napus（2n=38，AACC）and B. carinata（2n=34，BBCC），and they showed determinate inflorescence characteristic from B. carinata and the intermediate morphology of both parents. F1 hybrids showed the expected chromosomal composition（2n=36， ABCC）and eight B.carinata（referred B）chromosomes from by GISH analysis. Their pollen mother cell（PMCs） showed multiple chromosome pairing configuration at diakinesis of meiosis，with main segregation rate of 17： 19 at anaphase Ⅰ（AⅠ）. A number of lagging chromosomes and chromosomal bridges appeared during AⅠ and A Ⅱ，which caused pollen abortion. By continuous backcross using its parental line Zhongshuang 11（B.napus）， the BC5F1 lines with determinate inflorescence were obtained and they had similar morphology with Zhongshuang 11. Their somatic chromosomes（2n=38）showed normal programmed meiosis，and only minor B chromosome signals but whole chromosomes were detected by GISH analysis. By marker assisted detection using 46 pairs of B genome specific SSR primers，B genome specific bands are detectable in F1 hybrid and a few of BC5F1. This result indicated that the chromosomal segments of B.carinata were successfully introduced into B.napus L.Thus， a number lines with new determinate inflorescence will become useful in rapeseed breeding for new varieties suitable for mechanized operation.