A good-quality two-line male sterile line (Y58S) was crossed with a good-quality maintainer line (M215B) that carry multiple disease resistant genes. The obtained F1 hybrids were crossed with RGD-7S, which is a good-quality and disease-resistant male sterile line at the heading stage, a two-line male sterile line (He-9S) that contained five resistance genes ,Pi-2, Pi-1, Pi-Kh, Xa21 and Xa23 was bred by marker-assisted selection in combination with pedigree breeding. The average incidence rate of leaf blast in the field identification of He-9 S was only 2.5% (83.0% for the susceptible control), and the disease index of leaf blast was only 0.60 (52.9 for the susceptible control). The incidence rate of panicle neck blast of He-9 S was 38.3% (88.5% for the susceptible control) and the disease index of panicle neck blast was 8.0 (62.3 for the susceptible control) . The Frequency of resistance of He-9S in indoor artificial inoculation was 87.5%(6.25 for the susceptible control).Tests for rice blast resistance under greenhouse condition and field conditions showed moderate resistant to rice blast in He-9S. Furthermore, inoculation with three respective isolates (PXO99, ZHE173 and GD1358) showed that the diseased lesion length of He-9S was variable between 0.1-0.4 cm, similarly to the resistant donor parent, M215B, the lesion length of which were 0.1 cm, 0.1 cm and 0.3 cm respectively. On the contrast, the lesion length of both control varieties RGD-7S and Y58S were variable from 1.3 cm to 7.8 cm. Attractively, He-9S showed low fertility conversion temperature (about 23 °C), good versatility, high stigma exsertion rate and excellent rice quality. Up to date, >10 hybridization combinations have been produced by taking use of He-9S. For example, two elite variety Heliangyou 676 and Heliangyou 639, which exhibited moderate growth period ,high yield, good resistance and good stability, had been approved and released to farmers in May 2019 and on June 2020 respectively in Fujian province, and there are more than 3 varieties are in second-year tests or production test. Taken together, this study released an elite sterile line with a predictable potential in breeding for elite rice hybrids.