2025-5-6- 10
Home > Archive>Volume 21, Issue 4, 2020 >984-990. DOI:10.13430/j.cnki.jpgr.20191010003
PDF HTML XML Export Cite reminder
Molecular and Cytogenetic Identification of Wheat-Th. intermedium 6JS/6B Substitution Line
Author:
Affiliation:

1.College of Bio-engineering,Shanxi University;2.Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture;3.College of Life Science and Technology, University of Electronic Science and Technology of China;4.Millet Research Institute, Shanxi Academy of Agricultural Sciences

Fund Project:

National Key R&D Program of China (2016YFD0102004); the Key R&D Program of Shanxi Province (201803D221018-5, 201703D211007, 201803D421020); Shanxi Academy of Agricultural Sciences (YGG17123, YCX2018D2YS01); the Key Scientific and Technological Innovation Platform (201605D151002)

  • Article
    • | |
  • Metrics
    • |
  • Reference [29]
    • | | | |
  • Comments
    Abstract:

    Transforming resistance genes of wild relatives into elite cultivars is an effective approach to improve the wheat disease resistance. Identification of the alien chromosomes by deployment of a rapid and precise method becomes important in order to simplify the process on selection and utilization of the favorable genes. In this study, CH357, a new wheat germplasm resource which was derived from wheat-Thinopyrum intermedium partial amphiploid TAI7047, was subjected for tests upon infections of Powdery mildew and Stripe rust, as well as the evaluation by fluorescent in situ hybridization (FISH) and molecular markers detection. The results showed that CH357 contained a wheat-Th. intermedium 6JS/6B chromosome substitution. This genotype was tested to be resistant against wheat powdery mildew and stripe rust, and the resistances were likely caused by 6JS chromosome of Th. Intermedium, thus raising a potential for wheat resistance breeding. Furthermore, 160 STS markers were developed based on the Contigs sequences of Th. intermedium group 6 genome. Out of that, eight markers were found to specifically amplify the alien chromosome of CH357. The PCR-based assay will provide a user-friendly method for identifying the 6JS chromosome or fragments of Th. intermedium in wheat.

    Reference
    [1] 安调过, 许红星, 许云峰. 小麦远缘杂交种质资源创新. 中国生态农业学报,2011, 19(5): 1011-1019An D G, Xu H X, Xu Y F. Enhancement of wheat distant hybridization germplasm. Chinese Journal of Eco-Agriculture, 2011, 19(5): 1011-1019
    [2] 杨世湖, 徐利远, 刘大钧. 中间偃麦草染色体组型研究. 作物学报, 1988, 14(4): 279-283Yang S H, Xu L Y, Liu D J. A study on genome constitution of Elytrigia Intermedia(Host) Nevski. Acta Agronomica Sinica, 1988, 14(4): 279-283
    [3] 董玉琛. 小麦的基因源. 麦类作物学报, 2000, 20(3): 78-81Dong Y C. Genepools of common wheat. Journal of Triticeae Crops, 2000, 20(3): 78-81
    [4] 王洪刚, 刘树兵, 亓增军, 孔凡晶, 高居荣. 中间偃麦草在小麦遗传改良中的应用研究. 山东农业大学学报: 自然科学版, 2000, 31(3): 333-336Wang H G, Liu S B, Qi Z J, Kong F J, Gao J R. Application studies of Elytrigia intermedium in hereditary improvement of wheat. Journal of Shandong Agricultural University: Natural Science, 2000, 31(3): 333-336
    [5] Cauderon Y. Genome analysis in the genus Agropyron. Hereditas, 1966, 2: 218-234
    [6] 孙善澄. 小麦与偃麦草远缘杂交的研究. 华北农学报, 1987, 2(1): 7-12Sun S C. A study on the distant cross of wheat and agropyron. Acta Agriculturae Boreali-Sinica, 1987, 2(1): 7-12
    [7] Liu S B, Wang H G, Zhang X Y, Li X F, Li D Y, Duan X Y, Zhou Y L. Molecular Cytogenetic Identification of a Wheat-Thinopyron intermedium (Host) Barkworth DR Dewey Partial Amphiploid Resistant to Powdery Mildew. Journal of Integrative Plant Biology, 2005, 47(6): 726-733
    [8] Bao Y G, Li X F, Liu S B, Cui F, Wang H G. Molecular Cytogenetic Characterization of a New Wheat-Thinopyrum intermedium Partial Amphiploid Resistant to Powdery Mildew and Stripe Rust. Cytogenetic and genome research, 2009, 126(4): 390-395
    [9] 罗小军, 李欣, 乔麟轶, 郭慧娟, 贾举庆, 张树伟, 梅超, 畅志坚, 张晓军. 偃麦草属植物对小麦的遗传改良研究进展. 山西农业科学, 2019, 47(3): 482-485, 490Luo X J, Li X, Qiao L Y, Guo H J, Jia J Q, Zhang S W, Mei C, Chang Z J, Zhang X J. Research Progress of Wheat Genetic Improvement Derived from Elytrigia. Journal of Shanxi Agricultural Sciences, 2019, 47(3): 482-485, 490
    [10] 温辉芹,张立生,程天灵,李生海. 国审小麦新品种晋太170 的选育实践与体会. 山西农业科学,2008, 36(9): 15-20Wen H Q, Zhang L S, Cheng T L, Li S H. Breeding Practice and experience of the new strong gluten wheat variety of national authorized Jiantai170. Journal of Shanxi Agricultural Sciences, 2008, 36(9): 15-20
    [11] Liu C, Li G R, Gong W P, Han R, Li H S, Song J M, Liu A F, Cao X Y, Chu X S, Yang Z J, Huang C Y , Zhao Z D, Liu J J. Molecular and cytogenetic characterization of powdery mildew resistant wheat-Aegilopsmutica partial amphiploid and addition line. Cytogenetic Genome Research, 2016, 147(2-3):186-194
    [12] Hu L J, Li G R, Zeng Z X, Chang Z J, Liu C, Zhou J P, Yang Z J. Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance. Euphytica, 2011, 177(2): 169-177
    [13] Han F P,Lamb J C,Birchler J A. High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proceedings of the National Academy of Sciences of the USA, 2006, 103(9): 3238-3243
    [14] Tang Z X, Yang Z J, Fu S L. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics, 2014, 55(3): 313-318
    [15] Lang T, Li G R, Wang H J, Yu Z H, Chen Q H, Yang E N, Fu S L, Tang Z X, Yang Z J. Physical location of tandem repeats in the wheat genome and application for chromosome identification. Planta, 2019, 249(3): 663-675
    [16] Stewart C N, Via L E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR amplifications. Biotechniques, 1993, 14(5): 748-750
    [17] 刘淑娟, 张晓军, 李欣, 刘成, 白建荣, 任永康, 郑军, 李世姣, 郭慧娟, 梅超, 张树伟, 畅志坚, 乔麟轶. 中间偃麦草第6同源群特异STS标记开发. 草业学报, 2019, 28(4): 139-145Liu S J, Zhang X J, Li X, Liu C, Bai J R, Ren Y K, Zheng J, Li S J, Guo H J, Mei C, Zhang S W, Chang Z J, Qiao L Y. Development of specific STS markers for the sixth homologous group of Thinopyrum intermedium. Acta Prataculturae Sinica, 2019, 28(4): 139-145
    [18] Cui Y, Zhang Y P, Qi J, Wang H G, Wang R R C, Bao Y G, Li X F. Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes. Genome, 2018, 61(7): 515-521
    [19] Tsitsin N V. Remote hybridisation as a method of creating new species and varieties of plants. Euphytica, 1965, 14(3): 326-330
    [20] Kuraparthy V, Chhuneja P, Dhaliwal H S, Kaur S, Bowden R L, Gill B S. Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theoretical and Applied Genetics, 2007, 114(8): 1379-1389
    [21] 葛江燕, 陈士强, 高营营, 高勇, 朱雪, 黄泽峰, 陈建民. 基于抑制消减杂交开发长穗偃麦草(Lophopyrum elongatum)特异分子标记. 作物学报, 2012, 38(10): 1818-1826Ge J Y, Chen S Q, Gao Y Y, Gao Y, Zhu X, Huang Z F, Chen J M. Development of genome-specific molecular markers for Lophopyrum elongatum based on suppression subtractive hybridization. Acta Agronomica Sinica, 2012, 38(10): 1818-1826
    [22] 刘成, 杨足君, 刘畅, 李光蓉, 任正隆. 小麦族中含St染色体组物种的特异分子标记的建立. 遗传, 2007, 29(10): 1271-1279Liu C, Yang Z J, Liu C, Li G R, Ren Z L. Analysis of St-chromosome-containing triticeae polyploids using specific molecular markers. Hereditas: Beijing, 2007, 29(10): 1271-1279
    [23] Hu L J, Liu C, Zeng Z X, Li G R, Song X J, Yang Z J. Genomic rearrangement between wheat and Thinopyrum elongatum revealed by mapped functional molecular markers. Genes Genomics, 2012, 34(1): 67-75
    [24] Zhan H X, Zhang X J, Li G R, Pan Z H, Hu J, Li X, Qiao L Y, Jia J Q, Guo H J, Chang Z J, Yang Z J. Molecular characterization of anew wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust. International Journal of Molecular Sciences, 2015, 16(1): 2162-2173
    [25] Chen Q, Conner R L , Laroche A, Thomas J B. Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. 1998, 41(4):580-586
    [26] Stoutjesdijk P, Kammholz S J, Kleven S, Matsay S, Banks P M, Larkin P J. PCR-based molecular marker for the Bdv2 Thinopyrum intermedium source of barley yellow dwarf virus resistance in wheat. Australian Journal of Agricultural Research, 2001, 52(12): 1383-1388
    [27] Luo P G, Luo H Y, Chang Z J, Zhang H Y, Zhang M, Ren Z L. Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theoretical and Applied Genetics, 2009, 118(6): 1059-1064
    [28] He R L, Chang Z J, Yang Z J, Zhan H X, Zhang X J, Liu J X. Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theoretical and Applied Genetics, 2009, 118(6): 1173-1180
    [29] Liu J, Chang Z J, Zhang X J, Yang Z J, Li X, Jia J Q, Zhan H X, Guo H J, Wang J M. Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50, maps on wheat chromosome arm 4BL. Theoretical and Applied Genetics, 2013, 126(1): 265-274
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:1127
  • PDF: 1770
  • HTML: 0
  • Cited by: 0
History
  • Received:October 10,2019
  • Revised:November 15,2019
  • Adopted:December 12,2019
  • Online: July 20,2020
Article QR Code
You are the 634642th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.