Elite germplasm resources is the important foundation for the breeding of maize elite hybrid, and germplasm suitable for mechanical grain-harvesting is an urgent need in China. It is an important way to solve the problem by introducing European early maturity germplasm in domestic germplsm to develop Huanglv-European (HE) lines. In this study, the 258 HE lines were crossed with the tester lines Jing MC01 and Zheng 58 to evaluate the yield and grain moisture performances of the hybrids. The results indicated that: (1) the hybrids of Jing MC01 had lower grain moisture and higher grain yield as compared with the tester Zheng 58; (2) the Huanglv-European lines with different domestic background had much different performances in test crosses, Huangzao4 and Jing2416 derived lines had lower grain moisture and higher yield in both spring and summer maize, while the lines from Chang7-2 showed higher moisture and yield;(3) The hybrid from the HE lines with the percentage of 25% early maturity donors performed better in the target traits. Thus, the conclusion is that it would be an effective way to develop elite HE lines by backcrossing once with the domestic lines in practical breeding.