We previously cloned the full-length coding sequence of the gene AhMYB44，which showed a stress-induced expression from peanut drought transcriptome sequencing datasets. In order to investigate the function of AhMYB44，a yeast two-hybrid system was deployed for identifying its interacting proteins. The results showed that the bait vector pGBKT7-AhMYB44 was not toxic and showed no self-activation activity. Sixteen coding sequences were identified by screening of cDNA libraries which were constructed from a drought and high-salt stress of peanut. We further conducted an in-silico analysis of their homologous proteins，such as plant transcriptional regulation，growth and development，signal transduction，biotic and abiotic stress responses. Out of that，the candidate gene（No.46）was annotated with the abiotic stress，and encoded for a calcium-dependent phospholipid binding protein（ANNEXIN）. Therefore，we speculated that AhMYB44 maybe participate into regulation of peanut stress response process through intracellular calcium signal. This result might provide a reference for further research on the function and mechanism of AhMYB44.