Rice ‘Yuenong Simiao’ recently bred in Guangdong Province, China, with a strong resistance to rice blast, is a leading high-quality cultivar for regular cultivation, which can also be used as a hybrid rice restorer line. In this study, it has been revealed that ‘Yuenong Simiao’ has a broad spectrum of resistance against rice blast strains from South China. Genetic analysis of genetically segregated F2 population of ‘Yuenong Simiao’ × ‘LTH’ showed that the broad resistance spectrum of ‘Yuenong Simiao’ may be controlled by multiple genes. Using the molecular markers of major resistance genes Pi1, Pi2, Pi9, Pib and Pita, we have detected that ‘Yuenong Simiao’ contains at least Pi2 and Pib resistance loci. These results have revealed the cultivar’s genetic basis of high resistance to rice blast, and provides important molecular basis for extending the cultivation of disease-resistant cultivars.