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Home > Archive>Volume 21, Issue 3, 2020 >663-673. DOI:10.13430/j.cnki.jpgr.20191230002
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Genetic Dissection of Flag Leaf Related Traits and Grain Yield per Plant Using Genome-wide Association Analysis
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Affiliation:

1.Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/ Yangtze University, Jingzhou Hubei 434025;2.Laboratory of Lingnan Modern Agriculture/ Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen Guangdong 518210;3.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;4.Hubei Academy of Agricultural Sciences, Wuhan 430064

Fund Project:

Open fund of Engineering and Agricultural Use of Wetland, Ministry of Education (KF201806, KFT201901，KFT201905); The Agricultural Science and Technology Innovation Program Cooperation and Innovation Mission to JX（CAAS-ZDXT201800）

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    Abstract:

    Flag leaf, which is a major sink organ of rice plant, was reported to significantly positive correlate with the grain yield. Illustrating the genetic basis of flag leaf related traits and the grain yield is of significance in breeding for high-yield varieties with ideal plant type. In the present study, a diverse panel of 1016 rice accessions was evaluated for flag leaf related traits including flag leaf length (FLL), flag leaf width (FLW), flag leaf area (FLA), as well as the grain yield per plant (GY) in Jingzhou of Hubei province in two calendar years (2015 and 2016). The abundant variations at four traits were observed within this population, but the phenotypic variations were found to be largely environmental-dependent. The correlations between GY and each of FLL, FLW, FLA were low.Using genome-wide association analysis based on 4.8M high throughput SNP datasets, a total of 31 QTL for flag leaf related traits and GY were identified. Especially, one QTL (qFLW4) for FLW was detected in both two calendar years. Three regions were found to be associated with two traits including qFLW4 and qFLA4 for FLW and FLA, qFLL6 and qFLA6 for FLL and FLA, and qFLL10 and qFLA10 for FLL and FLA. Using haplotype and bioinformatic analyses in QTL regions, seven candidate genes and 10 favorable alleles were identified. Taken together, our results provided a preliminary base in order to clone the functional genes underlying rice flag leaf related traits, and breed for elite rice varieties with an ideal plant type by deploying different favorable genes by marker-assisted selection.

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History
  • Received:December 30,2019
  • Revised:January 14,2020
  • Adopted:February 03,2020
  • Online: May 18,2020
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