The resistance of five parthenocarpic cherry tomato materials against tomoto yellow leaf curl virus disease (TYLCVD) and gray leaf spot disease (GLSD) were identified using pathogen artificial inoculation and molecular markers. The genetic population was constructed using CP28, and the genetic law of parthenocarpy was studied. The ovary development process was observed at two different temperature environment (day/night temperature of 15°C/5°C and 25°C/15°C) using ultra thin paraffin section technology. The TYLCVD and GLSD parthenocarpic cherry tomato germplasm was constructed by regular hybridization and molecular markers. The results showed that no material was resistant to TYLCVD, but CP24, CR3 were resistant against GLSD. Based on the analysis of segregation of the genetic population, we concluded that the parthenocarpic trait of CP28 was controlled by recessive monogene. The paraffin sections showed no ovule in the ovary of tomato plants grown under low temperature (day/night temperature of 15°C/5°C), while some normal developed ovules were observed in tomato plants grown under normal temperature (day/night temperature of 25°C/15°C). Three new parthenocarpic germplasm resistant to both TYLCVD and GLSD were constructed, It laid a foundation for breeding of new cherry tomato varieties with parthenocarpy and multiple resistance.