2025-5-6- 21
Home > Archive>Volume 22, Issue 1, 2021 >139-148. DOI:10.13430/j.cnki.jpgr.20200226001
PDF HTML XML Export Cite reminder
Mapping of a New Quantitative Locus qPRO-19-1 Associated with Seed Crude Protein Content in Soybean（ Glycine max L.）
Author:
Affiliation:

1College of Life Sciences, Jilin Agriculture University, Changchun 130000; 2National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Soybean Biology in Beijing( MOA) /Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 3Crops Research Institute of Jiangxi Academy of Agricultural Sciences, Nanchang 330200; 4Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 5Soybean Research Institute of Jilin Academy of Agricultural Sciences, Changchun 130033

Fund Project:

National Natural Science Foundation of China(31960408)；The Agricultural Science and Technology Innovation Program

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Soybean is an economically-important food crop that contains about 40% of grain protein. The soybeans harvested from China are mainly used for food， and breeding for higher protein content is one of the main targets in soybean. Exploration of the genes related to soybean protein content and development of molecular markers are thus of great significance. In this study， a recombinant inbred line population was used by crossing a low protein soybean variety‘ ZH35’ （ female parent） with the high protein accession‘ Tokachi nagaha’ （ male parent） that was introduced from Japan. With the Bulked Segregant Analysis（ BSA） analysis， nine SSR markers that associated to protein content were identified on three chromosomes， including chromosome 19 where no QTL was previously reported. This QTL qPRO-19-1 was allocated using an inclusive complete interval mapping method（ ICIM-ADD） to the genetic interval of the molecular markers SSR_19_38 and SSR_19_59. This elite allele， which was derived from‘ Tokachi Nagaha’ ， could explain 7.81% and 14.87% of phenotypic variations in F2： 15and F2： 16 with the LOD values of 3.43 and 3.98， respectively. The physical interval of qPRO-19-1 expanded 385 kb that harbored 36 annotated genes. Collectively， the genetic mapping of the new QTL qPRO-19-1 laid the foundation for map-based cloning gene and molecular marker assisted breeding for high protein soybean varieties.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:February 26,2020
  • Revised:April 22,2020
  • Adopted:July 21,2020
  • Online: January 07,2021
Article QR Code
You are the th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.