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Home > Archive>Volume 22, Issue 1, 2021 >115-120. DOI:10.13430/j.cnki.jpgr.20200405001
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Diagnostic Marker Based Null LOX-1 Barley Germplasm Identification
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Affiliation:

1College of Agronomy, Gansu Agricultural University/Key Laboratory of Arid Land Crop Science in Gansu Provice/Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070; 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081

Fund Project:

the National Key R&D Program of China(2018YFD1000706-1)，the Conservation and Utilization for Crop Germplasm Resources(2019NWB036-06)，the China Agriculture Research System (CARS-05)

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    Abstract:

    The components， which are derived from the lipid metabolism of barley malt， destabilizes the beer flavor and its shelf life in the brewing industry. The lipoxygenase 1（ LOX-1） has been approved as the key enzyme involved in lipid metabolism in barley seeds. The identification and development of null LOX-1 barley germplasm might be of interest to promote high-quality malting barley breeding. By taking use of the previously-identified null LOX-1 Chinese barley rare allelic variation， here a Kompetitive Allele Specific PCR（ KASP） based diagnostic marker was generated and subjected for a test in 633 barley landraces which originated from Shandong and Henan province. A total of eight null LOX-1 barley accessions were identified， the geographic distribution and the allele frequency were further investigated. Taken together， this work provided LOX-1 free germplasm accessions valuable for the malting barley breeding especially in conjugation with the newly-generated KASP diagnostic

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History
  • Received:April 05,2020
  • Revised:April 25,2020
  • Adopted:May 06,2020
  • Online: January 07,2021
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