In this study, three members of the NPR1 family were identified by using the genomic information of Vernicia fordii (tung tree), and were named VfNPR1, VfNPR3 and VfNPR5. The physicochemical properties, subcellular localization, distribution of chromosomes, conservative domain, gene structure, and cis-acting regulatory elements were predicted through bioinformatic methods. The phylogenetic relationship of the NPR1 family in five species of the Euphorbiaceae and Arabidopsis thaliana was analyzed. To understand the expression patterns of the NPR1 family in flowers of different sexualities, in seeds at different phases of development, and in the root system of tung tree under the stress of Fusarium oxysporum, the transcriptional profiles were analyzed by use of the transcriptome sequencing data. The results showed that the NPR1 family members in the five species of the Euphorbiaceae were clearly divided into three subgroups (Clade Ⅰ, Clade Ⅱ, and Clade Ⅲ), and those in Ricinus communis, Jatropha curcas and tung tree showed obvious contraction. The important functional sites of npr1-1 (His334Tyr), npr1-2 (Cys150tyr) and nim1-2 (His300tyr) were quite conservative in the five species of the Euphorbiaceae. The three members of the NPR1 family in tung tree were hydrophobic proteins. VfNPR1, VfNPR3 and VfNPR5 were assigned into chromosomes 8, 10 and 4, respectively. VfNPR1 was located in nuclei, and VfNPR3 and VfNPR5 were located in chloroplasts. The three members of the NPR1 family were significantly differentially expressed in female, male and bisexual flowers, with the highest expression in male flowers. In different development phases of the tung tree seed, VfNPR1 and VfNPR5 had higher expression in the early phase (15 WAF and 10 WAF), while VfNPR3 had higher expression in the late phase (30 WAF). Under the stress of Fusarium oxysporum, the expression of the three members of the NPR1 family was significantly inhibited in the root system of tung tree seedlings at different times of infection, and VfNRP3 was completely inhibited in the late infection period. These results indicate that the three members of the NPR1 family may be involved in the flower and fruit development and the SAR signaling pathway of the root system in tung tree. This study provides important scientific basis for the high-yield cultivation techniques of tung tree, and also provides good genetic resources for the improvement of the tree disease resistance.