Thirty three tea plant resources from Mangshan in Hunan Province were investigated and collected, 24 of which were studied for biological characters and 27 of which were studied for quality analysis. The 24 resources were mainly small trees with upright habit. The young leaves were mostly glabrous or slightly pubescent, light green or yellow green in color, and the adult leaves were green or dark green, medium-sharply medium-dense- to sparse-serrated and flat at margin, with slightly raised upper surface, acuminate apex, and medium texture. The coefficient of variation of 12 characters ranged from 38.07% to 77.90%, with the average value of 58.70%, and the genetic diversity index was 0.51～1.05, with the average value of 0.81. The variation coefficient of 18 quality components of the 27 resources ranged from 4.86% to 71.32%, with an average of 28.29%; the diversity index ranged from 1.59 to 2.03, with an average of 1.86; the principal component analysis indicated that the cumulative contribution rate of the first 5 principal components was 84.36%. The resources were divided into 5 groups. Group I contained 15 resources, which were divided into two subgroups. Both Group II and Group III contained 5 resources, while Group IV and Group V contained 1 resource each. One low-caffeine and 3 high-caffeine tea plant resources were preliminarily screened out. This study provides a basis for the exploration and utilization of Mangshan tea plant resources and researches in the evolution of tea plants in Hunan Province.