Molecular Detection and Quality Properties Analysis of Waxy and HMW-GS in 47 Introduced Wheat Germplasms
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1.Center of Wheat Research, Henan Institute of Science and Technology;2.Xinke College, Henan Institute of Science and Technology

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The Key projects of Science and Technology of Henan Province (No.202102110161 );the Key Scientific Research Projects of Colleges and Universities in Henan Province (No.21A210007)

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    Abstract:

    In order to effectively utilize wheat germplasm introduced from abroad, Waxy and HMW-GS alleles were detected in 47 introduced wheat materials, and the quality parameters of amylose, amylopectin and wet gluten were analyzed. The results showed that there were three types of Wx-A1a, Wx-A1g and Wx-A1b at the Wx-A1 locus, and 39 materials (82.98%) were mainly of Wx-A1a type. Wx-B1a, Wx-B1e and Wx-B1b were located in the Wx-B1 locus, and 37 materials (78.72%) were mainly of Wx-B1a type. Wx-D1a and Wx-D1b were discovered at Wx-D1 locus, and 46 materials were of Wx-D1a type. A total of 8 Wx-1 allele combinations were identified, and 31 materials (65.96%) were mainly of Wx-A1a/B1a/D1a combination. The results also showed that the Glu-A1 alleles Ax2*, Null and Ax1 were distributed in 18, 18 and 11 materials, respectively. At the Glu-D1 locus, it was found that 23 (48.94%) and 21 (44.68%) materials contained Dx2 and Dx5, respectively; 22 (46.81%) and 20 (42.55%) materials contained Dy12 and Dy10, respectively; 2 materials possessed heterozygous Dy10+Dy12 type. A total of 19 kinds of Glu-A1/D1 allele combinations were identified, and Null/Dx5+Dy12 allele combinations were displayed in 7 materials. The quality analysis showed that the materials with Wx-A1a/B1a/D1a had relatively high amylose and low amylopectin contents, and the materials with Ax1 or Ax2* and Dx5+Dy10 possessed relatively high wet gluten content. Those results suggested that the introduced wheat germplasms were rich in Waxy and HMW-GS alleles, which would provide reference for rational utilization of germplasm resources and quality improvement of modern common wheat.

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History
  • Received:September 13,2020
  • Revised:December 08,2020
  • Adopted:December 18,2020
  • Online: March 09,2021
  • Published:
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