2025-6-8- 23
  • Home
  • About Journal
  • Editorial Board
  • Author
    Instruction
    Copyright Agreement
  • Ethcis Statement
  • Subscribe
  • Contact
  • 中文
Home > Archive>Volume 22, Issue 2, 2021 >371-379. DOI:10.13430/j.cnki.jpgr. 20200913001 Online First
PDF HTML XML Export Cite reminder
Molecular Detection and Quality Properties Analysis of Waxy and HMW-GS in 47 Introduced Wheat Germplasms
DOI:
10.13430/j.cnki.jpgr. 20200913001
CSTR:
Author:
  • HU Xi-gui 1

    HU Xi-gui

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • WU Xiao-jun 1

    WU Xiao-jun

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHANG Hai-hui 2

    ZHANG Hai-hui

    Xinke College, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • WANG Yu-quan 1

    WANG Yu-quan

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHANG Ran 1

    ZHANG Ran

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHAO Wei 1

    ZHAO Wei

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • RU Zhen-gang 1

    RU Zhen-gang

    Center of Wheat Research, Henan Institute of Science and Technology
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
Affiliation:

1.Center of Wheat Research, Henan Institute of Science and Technology;2.Xinke College, Henan Institute of Science and Technology

Clc Number:

Fund Project:

The Key projects of Science and Technology of Henan Province (No.202102110161 );the Key Scientific Research Projects of Colleges and Universities in Henan Province (No.21A210007)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference [29]
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    In order to effectively utilize wheat germplasm introduced from abroad, Waxy and HMW-GS alleles were detected in 47 introduced wheat materials, and the quality parameters of amylose, amylopectin and wet gluten were analyzed. The results showed that there were three types of Wx-A1a, Wx-A1g and Wx-A1b at the Wx-A1 locus, and 39 materials (82.98%) were mainly of Wx-A1a type. Wx-B1a, Wx-B1e and Wx-B1b were located in the Wx-B1 locus, and 37 materials (78.72%) were mainly of Wx-B1a type. Wx-D1a and Wx-D1b were discovered at Wx-D1 locus, and 46 materials were of Wx-D1a type. A total of 8 Wx-1 allele combinations were identified, and 31 materials (65.96%) were mainly of Wx-A1a/B1a/D1a combination. The results also showed that the Glu-A1 alleles Ax2*, Null and Ax1 were distributed in 18, 18 and 11 materials, respectively. At the Glu-D1 locus, it was found that 23 (48.94%) and 21 (44.68%) materials contained Dx2 and Dx5, respectively; 22 (46.81%) and 20 (42.55%) materials contained Dy12 and Dy10, respectively; 2 materials possessed heterozygous Dy10+Dy12 type. A total of 19 kinds of Glu-A1/D1 allele combinations were identified, and Null/Dx5+Dy12 allele combinations were displayed in 7 materials. The quality analysis showed that the materials with Wx-A1a/B1a/D1a had relatively high amylose and low amylopectin contents, and the materials with Ax1 or Ax2* and Dx5+Dy10 possessed relatively high wet gluten content. Those results suggested that the introduced wheat germplasms were rich in Waxy and HMW-GS alleles, which would provide reference for rational utilization of germplasm resources and quality improvement of modern common wheat.

    Key words:wheat; Waxy; HMW-GS; quality property
    Reference
    [1] Yamamori M, Nakamura T, Nagamine T. Polymorphism of two waxy proteins in the emmer group of tetraploid wheat, Triticum dicoccoides, T. dicoccum,and T. durum. Plant Breeding, 1995, 114(3): 215-218.
    [2] Nakamura T, Yamamori M, Hirano H, Hidaka S. Identification of three wx proteins in wheat (Triticum aestivum L.). Biochemical Genetics, 1993, 31(1/2): 75-86.
    [3] Nakamura T, Yamamori M, Hirano H, Hidaka S. Decrease of waxy (wx) protein in two common wheat cultivars with low amylose content. Plant Breeding, 1993, 111(2): 99-105.
    [4] Vrinten P, Nakamura T, Yamamori M. Molecular characterization of waxy mutations in wheat. Molecular Genetics and Genomicss, 1999, 261(3): 463-471.
    [5] Wickramasinghe H A M, Miura H. Gene dosage effect of the wheat Wx alleles and their interaction on amylose synthesis in the endosperm. Euphytica, 2003, 132(3): 303-310.
    [6] Jaksics E, Paszerbovics B, Egri B, Rakszegi M,Tremmel-Bede K, Vida G, Gergely S, Németh R, T?m?sk?zi S. Complex rheological characterization of normal, waxy and high-amylose wheat lines. Journal of Cereal Science, 2020, 93: 102982.
    [7] Payne P I, Lawrence G J. Catalogue or alleles for the complex gene loci,Glu-A1, Glu-B1 and Glu-D1 which code for the high-molecular-weight subunit of glutenin in hexaploid wheat. Cereal Research Communications, 1983, 11: 29-35.
    [8] Shewry P R, Halford N G, Tatham A S. High molecular weight subunits of wheat glutenin. Journal of Cereal Science, 1992, 15(2): 105-120.
    [9] Payne P I, Nightingale M A, Krattiger A F, Holt L M. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grownwheat varieties. Journal of the Science of Food and Agriculture, 1987, 40(1): 51-65.
    [10] Gupta G B, Macritchie F. Allelie variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheats.II. Biochemical basis of the allelic effects on dough properties. Journal of Cereal Science, 1994, 19(1): 19-29.
    [11] Popineau Y, Cornet M, Lefebvre J, Marchylo B. Influence of high Mr glutenin subunits on glutenin polymers and rheological properties of glutens and gluten subfractions of near-isogenic lines of wheat sicco. Journal of Cereal Science, 1994, 19(3): 231-241.
    [12] 贾继增. 分子标记种质资源鉴定和分子标记育种. 中国农业科学, 1996, 29(4): 1-10.
    Jia J Z. Molecular germplasm diagnostics and molecular marker -assisted breeding. Scientia Agricultura Sinica, 1996, 29(4): 1-10.
    [13] Liu S Chao S, Anderson J A. New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics, 2008, 118(1):177-183.
    [14] Vafin R, Abdulina I R, Rzhanova I V, Garaeva A L, Tyulkin S V, Askhadullin D F, Vasilova N Z, Zaynullin L I, Alimova F K, Shagalieva G I. Molecular approaches for identification of Tritictum aestivum L. genotypes by allelic variants of Waxy-genes and HMW glutenin subunits. Modern Problems of Science and Education, 2015, 2(1):13.
    [15] Morris C F,Kiszonas A M,Beecher B S, Peden G L. Registration of six partial waxy near-isogenic hexaploid wheat genetic stock lines lacking one or two granule bound starchsynthase I genes. Journal of Plant Registrations, 2020, 14(2):217–220.
    [16] Vafin R, Rzhanova I, Askhadullin D, Askhadullin D, Vasilova N. Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits. Acta Agrobotanica, 2018, 71(4): 1746.
    [17] Yan Z H, Wan Y F, Liu K F, Zheng Y L, Wang D W. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin, 2002, 47: 222-227.
    [18] Yamamori M, Quynh N T. Differential effects of Wx-A1, -B1 and -D1 protein deficiencies on apparent amylase content and starch pasting properties in common wheat. Theoretical and Applied Genetics, 2000, 100:32–38.
    [19] Nakamura T, Vrinten P, Saito M, Konda M. Rapid classification of partial waxy wheats using PCR-based markers. Gemome, 2002, 45:1150-1156.
    [20] Zhao X C, Batey I L, Sharp P J, Crosbie G, Barclay I, Wilson R, Morell M K, Appels R.. A single genetic locus associated with starch granule properties and noodle quality in wheat. Journal of Cereal Science, 1998, 27(1): 7-13.
    [21] Payne P I, Harris P A, Law C N, Holt L M, Blackman J A. The high-molecular-weight subunits of glutenin: structure genetics and relationship to bread-making quality. Annales De Technologie Agricole, 1980, 29(2): 309-320.
    [22] Lawrence G J, MacRitchie F, Wrigley C W. Dough and baking quality of wheat line deficient in glutenin subunits controlled by the Glu-A1, Glu-B1and Glu-D1 loci. Journal of Cereal Science, 1988, 7(2):109-112.
    [23] Dai S F, Xu D Y, Yan Y L, Wen Z J, Zhang J B, Chen H X, Lu Z F, Li H Y, Cong H, Wei Y M, Zheng Y L, Yan Z H. Characterization of high- and low-molecular-weight glutenin subunits from Chinese Xinjiang wheat landraces and historical varieties. Journal of Food Science and Technology, 2020, 57(10):3823-3835.
    [24] 王子宁, 郭北海, 李洪杰, 张艳敏,温之雨. 多倍体麦类作物Wx蛋白检测的SDS-PAGE方法. 遗传, 2000, 22(3): 169-171.
    Wang Z N, Guo B H, Li H J, Zhang Y M, Wen Z Y. SDS-PAGE for the detection of Wx protein in polyploid wheats. Hereditas, 2000, 22(3): 169-171.
    [25] Zhao X C, Sharp P J. An improved 1-D SDS–PAGE method for the identification of three bread wheat Waxy proteins. Journal of Cereal Science, 1996, 23(2):191-193.
    [26] 贾琳琳, 王永霞, 靳晓杰. 基于SDS-PAGE 与荧光标记检测技术的黄淮麦区小麦品种(系)HMW-GS 组成分析. 河南农业科学, 2019, 48(11):18-26.
    Jia L L, Wang Y X, Jin X J. HMW-GS composition analysis of wheat varieties(lines) in Huanghuai Region based on SDS-PAGE and fluorescence markers. Journal of Henan Agricultural Sciences, 2019, 48(11):18-26.
    Related
    Cited by
Get Citation

Copy
Related Videos

Share
0
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 13,2020
  • Revised:December 08,2020
  • Adopted:December 18,2020
  • Online: March 09,2021
  • Published:
Article QR Code
You are the th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.