2025-4-5- 21
Home > Archive>Volume 22, Issue 3, 2021 >782-788. DOI:10.13430/j.cnki.jpgr.20201108001
PDF HTML XML Export Cite reminder
Genetic Mapping of the Quantitative Trait Locus Contributes to the Grain Weight in Cultivar Yangmai13
Author:
Affiliation:

1.Lixiahe Institute of Agriculture Sciences / Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Yangzhou;2.Jiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian

Fund Project:

National Key Research and Development Program of China（2017YFD0100801）；Natural Science Foundation of Jiangsu Province (BK20171279)；National Natural Science Foundation of China (32071999)；Yangzhou Agricultural Research System（YZ2020033）

  • Article
    • | |
  • Metrics
    • |
  • Reference [27]
    • | |
  • Cited by
    • | |
  • Comments
    Abstract:

    Grain weight (GW) is one of key factors contributing the wheat yield. In wheat, breeding for higher GW becomes an important target. In this study, a population of 198 RILs crossed by C615 and Yangmai13 (YM13) was constructed, followed by genotyping with 90K SNP array and quantifying thousand grain weight at four environmental conditions. By using the genome-wide association scanning (GWAS), QTGW.yaas-1BL derived from C615 was detected in one environment at 12.90 cM on 1BL, which accounted for 3.07% of the phenotypic variances and 0.78 of the additive effect. The second locus QTGW.yaas-6AL derived from Yangmai13 was verified at four environments between 112.70 cM~116.00 cM on 6AL, accounting for 7.63%~10.55% of the phenotypic variances and 1.46~1.51 of the additive effect. Genotyping with functional KASP (Kompetitive allele-specific PCR) markers associating with the grain weight revealed six higher grain weight alleles commonly detected in C615 and YM13, and three alleles that are polymorphic between parents. Collectively, the new grain weight QTL identified will lay the foundation for the genetic studies of grain weight in YM13 and in breeding for high-yielding wheat cultivars.

    Reference
    [1] 张福彦,范家霖, 陈晓杰, 陈锋, 齐红志, 王嘉欢, 程仲杰, 杨保安, 张建伟.小麦粒重相关基因的遗传定位和分子标记辅助育种进展. 植物遗传资源学报, 2020, 21(3): 507-516.Zhang F Y, Fan J L, Chen X J, Chen F, Qi H Z, Wang J H, Cheng Z J, Yang B A, Zhang J W. Genetic localization and marker assisted breeding of grain weight-related genes in common wheat[J]. Journal of Plant Genetic Resources, 2020, 21(3): 507-516.
    [2] 曹廷杰, 赵虹, 王西成, 崔党群, 詹克慧. 河南省半冬性小麦品种主要农艺性状的演变规律. 麦类作物学报, 2010, 30(3): 439-442.Cao T J, Zhao H, Wang X C, Cui D Q, Zhan K H. Evolution of main agronomic traits for semi winter wheat varieties in Henan province. Journal of Triticeae Crops, 2010, 30(3): 439-442.
    [3] 宋健民, 戴双, 李豪圣, 程敦公, 刘爱峰, 曹新有, 刘建军, 赵振东. 山东省近年来审定小麦品种农艺和品质性状演变分析. 中国农业科学, 2013, 46(6): 1114-1126.Song J M, Dai S, Li H S, Cheng D G, Liu A F, Cao X Y, Liu J J, Zhao Z D. Evolution of agronomic and quality traits of wheat cultivars released in Shandong province recently. Scientia Agricultura Sinica, 2013, 46(6): 1114-1126.
    [4] Wu W, Li C J, Ma B L, Shah F, Liu Y, Liao Y C. Genetic progress in wheat yield and associated traits in China since 1945 and future prospects. Euphytica, 2014, 196(2): 155-168.
    [5] Wang Y Q, Hao C Y, Zheng J, Ge H M, Zhou Y, Ma Z Q, Zhang X Y. A haplotype block associated with thousand kernel weight on chromosome 5DS in common wheat (Triticum aestivum L.). Journal of Integrative Plant Biology, 2015, 57: 662-672.
    [6] Huang X Q, Coster H, M. Ganal W, DER M S R. Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2003, 106: 1379-1389.
    [7] Elouafi I, Nachit M M. A genetic linkage map of the Durum×Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theoretical and Applied Genetics, 2004, 108: 401-413.
    [8] Quarrie S A, Steed A, Calestani C, Semikhodskii A, Lebreton C, Chinoy C, Steele N, Pljevljakusi D, Waterman E, Weyen J, Schondelmaier J, Habash D Z, Farmer P, Saker L, Clarkson D T, Abugalieva A, Yessimbekova M, Turuspekov Y, Abugalieva S, Tuberosa R. A high-density genetic map of hexaploid wheat (Triticum aestivum L.) from the cross Chinese Spring × SQ1 and its use to compare QTLs for grain yield across a range of environments. Theoretical and Applied Genetics, 2005, 110: 865.
    [9] 苗永杰, 阎俊, 赵德辉, 田宇兵, 阎俊良, 夏先春, 张勇, 何中虎. 黄淮麦区小麦主栽品种粒重与籽粒灌浆特性的关系. 作物学报, 2018, 44(2): 260-267.Miao Y J, Yan J, Zhao D H, Tian Y B, Yan J L, Xia X C, Zhang Y, He Z H. Relationship between grain filling parameters and grain weight in leading wheat cultivars in the Yellow and Huai rivers valley. ACTA AGRONOMICA SINICA, 2018, 44(2): 260-267.
    [10] Wang X, Dong L, Hu J, Pang Y, Hu L, Xiao G Ma X, Kong X Jia J Wang H, Kong L. Dissecting genetic loci affecting grain morphological traits to improve grain weight via nested association mapping. Theoretical and Applied Genetics, 2019, 132(11): 3115-3128.
    [11] Liao X Z, Wang J, Zhou R H, Ren Z L, Jia J Z. Mining favorable alleles of QTLs conferring thousand-grain weight from synthetic wheat. Acta Agronomica Sinica, 2008, 34(11): 1877-1884.
    [12] Khalid M, Afzal F, Gul A, Amir R, Subhani A, Ahmed Z, Mahmood Z, Xia X, Rasheed A, He Z. Molecular characterization of 87 functional genes in wheat diversity panel and their association with phenotypes under well-watered and water-limited conditions. Frontiers in Plant Science. 2019, 10: 717.
    [13] Stacey J, Isaac P G. Isolation of DNA from plants. Methods in Molecular Biology. 1994, 28: 9-15.
    [14] Rasheed A, Wen W E, Gao F M, Zhai S N, Jin H, Liu J D, Guo Q, Zhang Y J, Dreisigacker S, Xia X C, He Z H. Development and validation of KASP assays for genes underpinning key economic traits in bread wheat. Theoretical and Applied Genetics, 2016, 129: 1843–1860.
    [15] 朱冬梅,胡文静,别同德, 等. 利用四交RIL群体定位小麦籽粒脱水速率QTL. 麦类作物学报, 2020, 40(1): 49-54.Zhu D M, Hu W J, Bie T D, Lu C B, Zhao R H, Gao D R. QTL mapping for kernel dehydration rate after physiological maturity using four-way RIL population of wheat. Journal of Triticeae Crops, 2020, 40(1): 49-54.
    [16] Stam. Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant Journal, 1993, 3: 739-744.
    [17] Wang S, Wong D, Forrest K, Allen A, Chao S, Huang B. E, Maccaferri M, Salvi S, Milner S G, Cattivelli L, Mastrangelo A M, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing, ConsortiumLillemo M, Mather D, Appels R, Dolferus R.Brown-Guedira G, Korol A, Akhunova A R, Feuillet C, Salse J, Morgante M, Pozniak C, Luo M C, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards K J, Hayden M, Akhunov E. Characterization of polyploidy wheat genomic diversity using the high density 90,000 SNP array. Plant Biotechnology Journal, 2014, 12: 787-796.
    [18] 王建康. 数量性状基因的完备区间作图方法. 作物学报, 2009, 35: 239-245.#$NLWang J K. Inclusive composite interval mapping of quantitative trait genes. Acta Agronomica Sinica, 2009, 35: 239-245.
    [19] 胡文静, 张勇, 陆成彬, 王凤菊, 刘金栋, 蒋正宁, 王金平, 朱展望, 徐小婷, 郝元峰, 何中虎, 高德荣. 小麦品种扬麦16赤霉病抗扩展QTL定位及分析. 作物学报, 2020, 46(2): 157-165.Hu W J, Zhang Y, Lu C B, Wang F J, Liu J D, Jiang Z N, Wang J P, Zhu Z W, Xu X T, Hao Y F, He Z H, Gao D R. Mapping and genetic analysis of QTLs for Fusarium head blight resistance to disease spread in Yangmai 16. Acta Agronomica Sinica, 2020, 46(2): 157-165.
    [20] 李慧慧, 张鲁燕, 王建康. 数量性状基因定位研究中若干常见问题的分析与解答[J]. 作物学报, 2010, 36: 918.#$NLLi H H, Zhang L Y, Wang J K. Analysis and answers to frequently asked questions in quantitative trait locus mapping[J]. Acta Agronomica Sinica, 2010, 36: 918.
    [21] Su Z Q, Hao C Y, Wang L F, Dong Y C, Zhang X Y. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2011, 122: 211.
    [22] 韩利明, 杨芳萍, 夏先春, 阎俊, 张勇, 曲延英, 王忠伟, 何中虎. 株高、粒重及抗病相关基因在不同国家小麦品种的分布. 麦类作物学报, 2011, 31(5): 824-831.Han L M, Yang F P, Xia X C, Yan J, Zhang Y, Qu Y Y, Wang Z W, He Z H. Distribution of genes related to plant height, kernel weight and disease resistance among wheat cultivars from major countries. Journal of Triticeae Crops, 2011, 31(5): 824-831.
    [23] Zhang X Y, Chen J S, Shi C L, Chen J N,Zheng F F, Tian J C. Function of TaGW2-6A and its effect on grain weight in wheat (Triticum aestivum L.). Euphytica, 2013, 192: 347-357.
    [24] 寇程, 李学军, 高欣, 吕千, 杜登峰, 程显, 李扬, 李立群. 小麦粒重基因TaGW2-6A等位变异的高通量分子检测. 麦类作物学报, 2015, 35(11):1467-1475.Kou C, Li X J, Gao X, Lv Q, Du D F, Cheng X, Li Y, Li L Q. High-throughput molecular detection of allelic variation in TaGW2-6A gene of wheat (Triticum aestivum L.). Journal of Triticeae Crops, 2015, 35(11): 1467-1475.
    [25] Sestili F, Pagliarello R, Zega A, Saletti R, Pucci A, Botticella E Masci S, Tundo S, Moscetti I, Foti S Lafiandra D. Enhancing grain size in durum wheat using RNAi to knockdown GW2 genes Theoretical and Applied Genetics, 2019, 132(2): 419-429.
    [26] Cao S H, .Xu D A, Hanif M, Xia X C, He Z H. Genetic architecture underpinning yield component traits in wheat. Theoretical and Applied Genetics, 2020, 133(6): 1811-1823.
    [27] 胡文静, 朱冬梅, 别同德, 陆成彬, 高德荣. 扬麦16籽粒灌浆速率相关性状的QTL定位(小麦15K SNP芯片法) 麦类作物学报, 2020, 40(8): 915-920.Hu W J, Zhu D M, Bie T D, Lu C B, Gao D R. Detection of QTLs for Grain-Filling rate related traits of Yangmai 16 based on 15K SNP array. Journal of Triticeae Crops, 2020, 40(8): 915-920.
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:1026
  • PDF: 2325
  • HTML: 0
  • Cited by: 0
History
  • Received:November 08,2020
  • Revised:January 04,2021
  • Adopted:February 03,2021
  • Online: May 07,2021
Article QR Code
You are the 605499th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.