Awn in common wheat is one of the important spike organs and plays a critical role in photosynthesis, insect resistance, bird-preventing and yield formation. The genetic basis on its formation and development remains largely unclear. To identify novel genetic loci involved in awn development, a genome-wide association study (GWAS) was performed using a diversity panel of 206 wheat accessions from Huang Huaihai region based on a Wheat 90K SNP array. The results showed that SNPs associated with phenotypic variation were detected on chromosomes 1A, 2A, 2B, 5A and 7A. SNPs with the highest significance were mainly located on chromosome 5A. Moreover, the bulked segregant analysis (BSA) in a recombinant inbred line population were analyzed using Wheat 660K SNP array. Out of 500 polymorphic SNPs, 158 were found in the 696.6-706.6 Mb interval of chromosome 5A, where the previously reported B1 locus encoding for Tipped1 was resided on the gene annotation of Chinese Spring. Gained from the re-sequencing result of this gene in parental lines, the wheat awn may be associated with a 25-bp insertion/deletion polymorphism located at -346 bp and a linkage SNP at -284 bp in the promoter region. Collectively, this study provided information for understanding the genetic mechanism of wheat awn development and molecular breeding.