SQUAMOSA promoter binding protein-like(SPL)gene family, as a class of transcription factors present widely in plants, has been shown to play important biological roles in plant growth, development, signal transduction, physiological and biochemical processes. In this study, 26 SPL genes from the radish genome have been identified using bioinformatic approach, and they are designated RsSPL1-RsSPL26 according to their locations on eight chromosomes. The amino acids encoded by SPL genes are variable from 139 to 1021 aa, with the protein molecular weight ranged from 16,167.7 to 112,219.48 Da and an isoelectric point of 5.77 to 9.67. Radish SPL genes contain 2 to 11 exons. MicroRNA target prediction suggested that 12 RsSPL genes contained complementary sequence of miR156 and 11 RsSPL genes contained complementary sequence of miR157. The expression patterns of RsSPL genes in different tissues and development stages showed spatial and temporal differences, and the same subfamily members showed similar expression patterns. Thus, these results provided insights for the functional characterization of the SPL genes family in radish.