Ear rot is a serious disease of maize caused by fungus in the producing areas. Discover excellent resistant germplasm resources and breeding resistant varieties are the most economical and effective measures to control maize ear rot. In this study, the ear rot resistance of 324 maize germplasm against Fusarium graminearum was identified by the ear puncture injection method in Tongzhou and Xiaotangshan of Beijing. Correlation analysis showed that there was a positive correlation between resistance evaluations in the two environments. According to the comprehensive analysis, 9 inbred lines with high resistance were OQ603, Bai Huang-hun, PI 539921, Mo17, PI 406119, F321, X178, B09 and PHG39, respectively. At the same time, it was found that the resistance of 48 inbred lines was above rated R level (including HR and R) in both breeding site. According to the resistance distribution of maize germplasm in different group, P group showed strong resistance overall, followed by Iodent and X germplasm, while the resistance distribution trend was not obvious in other populations. The identification of these resistant materials enriched the germplasm resources of maize to ear rot in China, and provided important materials for disease resistance breeding and theoretical research.