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Home > Archive>Volume 22, Issue 5, 2021 >1430-1441. DOI:10.13430/j.cnki.jpgr.20210218004
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Molecular Cloning and Functional Analysis of FtNAC11 from Tartary Buckwheat (Fagopyrum tataricum)
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Affiliation:

1.College of Pastoral Agriculture Science and Technology, Lanzhou University/State Key Laboratory of Grassland Agro-ecosystems;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences

Fund Project:

The National Key Research and Development Program of China (2019YFD1001300, 2019Y FD1001302)

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    Abstract:

    NAC (NAM, ATAF1/2, CUC2) gene plays an important role in responses to plant abiotic stresses. In this study, we cloned FtNAC11 from tartary buckwheat cultivar ‘Pinku No.1’. Sequence analysis showed that the full-length coding sequence of FtNAC11 was 774bp encoding for 257 amino acids residues, with a molecular weight of 8954.58 Da and an isoelectric point of 5.91.The sequence alignment and phylogenetic tree analysis showed that FtNAC11 had higher homology with Arabidopsis thaliana AtNAC61. The expression of FtNAC11 gene was induced under PEG or NaCl treatments. Sub-localization by transient expression and yeast transactivation assay indicated that FtNAC11 encoded for a nuclear transcription activator with an activation domain at the region of C-terminal. Moreover, the coding sequence of FtNAC11 was transformed into Arabidopsis thaliana by Agrobacterium-mediated transformation, and the transgenic plants over-expressing this gene resulted in significant enhancement under drought and salt tolerance treatments. Collectively, these results indicated that FtNAC11 is likely involved in abiotic stress adaptation, providing a basis for further investigation of its molecular mechanism responding to drought and salt tolerance in tartary buckwheat.

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History
  • Received:February 18,2021
  • Revised:April 16,2021
  • Adopted:April 27,2021
  • Online: September 07,2021
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