Autophagy pathway is known to be widely involved in the regulation of growth and development, nutrient recycle, a variety of biotic or abiotic stresses tolerance in plant. However, the functional research in rice is still in its infancy. In this study, OsATG12, a rice autophagy-related gene, was isolated and subjected for producing over-expressing and gene editing plasmids transformed into rice. We identified the transgenic lines over-expressing OsATG12 and atg12s mutant lines. By testing for rice blast resistance using these transgenic lines, OsATG12 was found to negatively regulate the blast resistance of rice. Collectively, this study provided new insights for revealing the functions of rice autophagy pathway and autophagy related genes.