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Home > Archive>Volume 22, Issue 6, 2021 >1651-1658. DOI:10.13430/j.cnki.jpgr.20210423001 Online First
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Genetic Identification and Preliminary Mapping of a Resistance Gene Against Southern Rice Black Streaked Dwarf Virus
DOI:
10.13430/j.cnki.jpgr.20210423001
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  • LI Yan-fang 1

    LI Yan-fang

    Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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  • FENG Fang 2

    FENG Fang

    Wuhan Greenfafa Institute of Novel Genechip Research and Development Co Ltd
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  • XIAO Han-xiang 1

    XIAO Han-xiang

    Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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  • YUAN Long-yu 1

    YUAN Long-yu

    Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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  • ZHOU Bo-quan 3

    ZHOU Bo-quan

    Zhuhai Modern Agricultural Development Center
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  • QIU Shu-qing 2

    QIU Shu-qing

    Wuhan Greenfafa Institute of Novel Genechip Research and Development Co Ltd
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  • ZHANG Yang 1

    ZHANG Yang

    Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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Affiliation:

1Plant Protection Research Institute Guangdong Academy of Agricultural Sciences,Guangzhou 510640; 2 Wuhan Greenfafa Institute of Novel Genechip Research and Development Co. Ltd,Wuhan 430064; 3 Zhuhai Modern Agricultural Development Center,Zhuhai 519000

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Fund Project:

Breeding new rice varieties with super high yield and tolerance to adversities (2018B020202004),Rice innovation team of modern agricultural technology system in Guangdong Province(2020KJ105)

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    Abstract:

    Southern rice black-streaked dwarf virus disease (SRBSDVD) is one of the most serious viral diseases. Exploration of resistant germplasm resource and identification of resistance-conferring gene become of interest in rice breeding. Here, by testing for SRBSDVD resistance in a breeding population by artificial inoculation under both field and greenhouse conditions followed by RT-PCR detection, individual plants showing a segregation on resistance and susceptibility were observed implying the presence of a new recessive virus resistance locus. We assembled the resistance and susceptibility bulks by pooling the resistant and susceptible plants, respectively. By genotyping using the whole genome breeding gene chip (GSR 40K) technology, this resistance gene against southern rice black-streaked dwarf virus disease was delimited in a lower resolution. Collectively, this study provided a resistance resource/gene valuable in resistance breeding against southern black streaked dwarf disease.

    Key words:SRBSDVD;gene chip;bulk segregant analysis(BSA);resistance identification
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History
  • Received:April 23,2021
  • Revised:July 15,2021
  • Adopted:July 19,2021
  • Online: November 04,2021
  • Published:
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