HU Meng-die
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityLI Jia-wei
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityWANG Mei
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityLIU Bin
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityMA Yu-cong
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityZHAO Yu-lu
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityYANG Xin-lei
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityCUI Shun-li
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityHOU Ming-yu
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural UniversityJIANG Xiao-xia
Hebei Yiyuan Ecological Agriculture Science and Technology Co LtdMU Guo-jun
National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural University1.National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural University;2.Hebei Yiyuan Ecological Agriculture Science and Technology Co Ltd
This research was funded by Key project of science and technology research in colleges and universities of the department of education in Hebei Province (ZD2019051); Key project of science and technology research of modern seed industry of the department of S&T in Hebei Province(19226363D);Construction of modern agricultural garden in Yixian county(2111N004).
Peanuts are an important product as part of the world's cereals, oils and foods. The testa colors of peanuts are white, red, purple, pink, and variegated. Variegated peanut testa are unique and can be distinguished from other types. In this study, the variegated testa peanut VG-02 was adopted as the research material. The study showed that the differentially expressed miRNA-enriched metabolic pathways related to the color synthesis included phenylpropane biosynthesis, flavonoid biosynthesis, isoflavone biosynthesis and rhythm-plant. MiRNA sequencing results showed that 86 miRNAs were differentially expressed of which 20 miRNAs were related to the color synthesis in variegated testa peanut. Twenty differentially expressed miRNAs related to anthocyanin synthesis in variegated testa peanut including miR_8, miR_50, miR_51 and miR_239-x, that jointly targeted anthocyanins, anthocyanidins and IFS target gene. Five miRNAs were found to targete structural genes in the anthocyanin biosynthesis: miR_398-x which regulates CHS target genes, miR_482 which regulates 4CL target genes, miR_266 and miR_182 which regulate F3'H target genes and miR_5 which regulates the anthocyanin 3-O-glucoside target gene. MiR858-y is a miRNA targeting anthocyanin biosynthesis regulation gene, which targets and regulats MYB2 and MYB3. MiR_10, miR_15, miR_61, miR_72, miR_102, miR_116, miR_123, miR_193, miR_256 and miR_862-z target CYP450 target genes.The joined analysis of KEGG metabolic pathways by miRNA sequencing and transcriptome analysis indicated that flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. This study further our understanding of the molecular mechanisms of anthocyanin synthesis in variegated testa peanut.