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Home > Archive>Volume 22, Issue 5, 2021 >1348-1354. DOI:10.13430/j.cnki.jpgr. 20210531001
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QTL Mapping for Wheat Powdery Mildew Resistance at the Adult Stage
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Affiliation:

Agricultural Bio-Technology Centre, Ningxia Academy of Agriculture and Forestry Sciences

Fund Project:

National Natural Science Foundation of China (31660394, 31960428)，Ningxia Hui Autonomous Region Natural Science Foundation of China (2019AAC03151, 2018AAC3189)，Ningxia Hui Autonomous Region Agricultural Breeding Program (2018NYYZ0202)，Ningxia Youth Talents Supporting Program (TJGC2019074)

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    Abstract:

    This study reported a quantitative trait locus (QTL) mapping for identifying the adult-stage powdery mildew resistance genes in a recombinant inbred line (RIL) population of common wheat (Triticum aestivum L.). This population was derived from the highly susceptible variety Ningchun 4 crossing with the highly resistant variety Ningchun 27, and genotyped using genotyping-by-sequencing (GBS) approach. The results showed that the additive-epistatic genetic model using four pairs of major genes was suitable for identifying the powdery mildew resistance loci. Two resistance-conferring loci QPm.naafs-4D and QPm.naafs-7D have been identified from Ningchun 27 and Ningchun 4, respectively. Out of them, QPm.naafs-4D represented a LOD value of 5.9 to 15.4 at multiple environmental conditions (additive effects: -1.1 to -1.2), and contributed to a phenotypic variation of 18.6% to 34.5%. This locus was delimited to a genetic distance of 4 cM harboring the durable poly-resistance gene Pm46/Lr67/Yr46/Sr55. Sequence analysis confirmed that QPm.naafs-4D in Ningchun 27 was identical to Pm46/Lr67/Yr46/Sr55. The resistance-conferring donor line is valuable in marker-assisted breeding for powdery mildew resistance in wheat.

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History
  • Received:May 31,2021
  • Revised:May 31,2021
  • Adopted:June 01,2021
  • Online: September 07,2021
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