Soybean cyst nematode（SCN）race 2 is predominant in the Huang-huai Rivers valley. Out of 333 soybean accessions that have been tested for the resistance to SCN race 2（SCN 2）since 2017，19 resistant accessions including 5 resistant and 14 moderately resistant have been identified. Genotyping using KASP markers showed that all the 19 resistant accessions harbored two resistant loci rhg1 and Rhg4. The genotypes of KASP markers linked to rhg1 and Rhg4 for 18 accessions were the same as PI90763，while Yuanheidou was consistent with PI437654. Moreover，resistant genotype Zheng 12915 and Zheng 12929，which were developed from resistant germplasm Huipizhiheidou showed agronomic performance better than the parental line. Collectively， these results provided a basis for SCN breeding in soybean.