Detection of Quality-Related Genes in the Wheat Landrace in Shanxi Province by KASP Markers
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Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops

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Shanxi Province General Youth Fund Project(201801D221313),Shanxi Academy of Agricultural Sciences Agricultural Science and Technology Innovation Research Project(YCX2020YQ48),Key Research and Development Projects in Shanxi Province(201903D211003)

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    Abstract:

    To explore and utilize the genetic resources of good quality traits in wheat landraces,15 quality trait-related genes in 405 wheat landraces of Shanxi province were genotyped using 30 KASP markers. The results showed that the average frequency of the favorable allelic variations Pinb-D1b and Pinb-B2b for grain hardness accounted for 4.94% and 63.46%,respectively. At the Glu-1 locus,the favorable alleles Glu-Ax1(or Glu-Ax2* ),Glu-Bx7OE and Glu-D1d accounted for 93.09%,47.16% and 0.74%,respectively. Elite alleles for flour color including, Psy-A1b,Psy-B1a(or Psy-B1b),Psy-D1a,TaPds-B1b,Zds-A1a,TaLcy-B1b,Lox-B1a,TaPod-A1b,Ppo-A1b and Ppo-D1a accounted for 1.23%,95.80%,87.65%,0.74%,87.16%,96.05%,88.89%,1.73%,97.28% and 20.94%, respectively. The favorable allele frequncy of landraces,which were derived from the late-maturing winter wheat region in central Shanxi and the south mid-maturing winter wheat region,ranged from 0.52% to 97.41% and 0 to 97.51%,respectively. In addition,nine favorable alleles Pinb-B2b/Glu-Ax1(or Glu-Ax2* )/Glu-Bx7OE/Psy-B1a (or Psy-B1b)/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b were simultanously detected in 89 samples,with a frequency of 21.98%. By conducting marker-assisted identification of the excellent alleles in wheat landraces,this study provided reference for the genetic improvement at quality-related traits in bread wheat.

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History
  • Received:September 10,2021
  • Revised:January 03,2022
  • Adopted:January 28,2022
  • Online: May 11,2022
  • Published:
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