2025-5-22- 9
Home > Archive>Volume 23, Issue 2, 2022 >527-540. DOI:10.13430/j.cnki.jpgr.20210912001
PDF HTML XML Export Cite reminder
Identification and Analysis Lignin Biosynthesis Genes of Averrhoa carambola
Author:
Affiliation:

1.College of Landscape Architecture, Fujian Agriculture and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization;2.Horticulture Research Insistute of Guangxi Academy of Agricultural Science

Fund Project:

Excellent Master Degree Thesis Fund of Fujian Agriculture and Forestry University (1122YS01010), The Key Research and Development Program of Guangxi (Gui Sci. AB16380134), Guangxi Academy of Agricultural Sciences Special Project for Basic Scientific Research (Gui Agri Sci. 2021YT046), Technology Innovation Special Fund Project of Fujian Agriculture and Forestry University (KFA17331A), Team of Flower Industry Expert Guidance and Service (1891008001)

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Lignin is the key component in the secondary cell wall of plants，which provides mechanical support and promotes water transport as well as has important impact responding to various biological and abiotic stresses. Averrhoa carambola L. is an important economic and ornamental species，the growing zone of which is restricted by its sensitivity to environmental stress conditions，such as strong wind，cold，and drought. However，lignin has significant impact on the improvement of stress resistance.To explore the lignin biosynthesis process of A. carambola L.，the issue microstructure was visualized using paraffin sections，and the changes of lignin content and enzyme activity were measured at different development stages of twigs. Bioinformatic analyses based on genome sequencing and transcriptome profiling were conducted to identify structural genes and transcription factors that associated to the lignin biosynthesis. The results showed that the increase on the activity of three enzymes（PAL，CAD and POD）and on the lignin content，as well as the appearance of xylem cells were observed along with the twigs development. A total of 41 structural genes，transcription factors（12 AcaMYBs and 11 AcaNACs）were identified in the A. carambola L. genome，followed by analyzing the differential expression patterns of these genes. The results of qRT-PCR found that AcaPAL1，AcaC3H2，AcaC4H2，AcaF5H1 and AcaMYB18 exhibit obvious tissue specific expression pattern，indicating these are specific genes related to lignin biosynthesis. Co-expression network analysis showed that AcaNAC29，AcaNAC39 and AcaMYB47were the essential regulatory genes of lignin biosynthesis. Collectively，this study provided important information to facilitate future research on the transcription factors and structural genes of lignin biosynthesis in A. carambola L..

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 12,2021
  • Revised:September 25,2021
  • Adopted:October 08,2021
  • Online: March 10,2022
Article QR Code
You are the th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.