Wheat（Triticum aestivum L.）is an important food crop worldwide. However，its growth has been heavily suppressed by salt stress. The molecular mechanisms of salt tolerance in wheat remain unclear. In this study we conducted research to decipher the mechanism of salt stress response in Jimai19 using NaCl treatment and time-course RNA sequencing. The total number of differentially expressed genes（DEGs）in response to salt stress was 5526 in wheat roots. Based on Gene Ontology（GO）and Kyoto Encyclopedia of Genes and Genomes （KEGG）analysis，it was found that DEGs were significantly enriched in the categories of plant hormone signal transduction，primary metabolic processes such as amino acid synthesis，and some secondary metabolic processes in the early stages of salt treatment. After 6 h of salt treatment，the DEGs involved in stress response began to enrich. With the extension of stress time and the damage to plant increased，the DEGs related to macromolecular complex，DNA conformational changes，protein-DNA structural changes etc，were enriched at 48 h and 72 h of salt treatment. Many genes involved in signal transduction，resistance to oxidative stress，osmotic stress，ion balance and amino acid sythesis were differentially expressed at different stages of salt treatment. Most genes of the last three classes were induced by salt stress. These results provided valuable information on the salt tolerance of molecular mechanisms in wheat.