To study the transcriptional regulation mechanism of SpsLAZY1a and SpsLAZY1b genes， their promoter fragments were cloned from Salix psammophila C. Wang & C. Y. Yang. By analyzing cisacting element in the promoter sequences using plantCARE database，the promoter sequences of both genes contain the core elements CAAT-box and TATA-box，and the elements responding to light，methyl jasmonate， abscisic acid，ethylene，gibberellin，and low temperature. GUS staining in tobacco transient expression and stable transgenic 84K poplar revealed the transcriptional activity of both promoters ProSpsLAZY1a and ProSpsLAZY1b. The ProSpsLAZY1a activity was stronger than that of ProSpsLAZY1b in transgenic 84K poplar. The results of stem basal sections of transgenic 84K poplar showed that the expression of ProSpsLAZY1a and ProSpsLAZY1b was mainly observed in endothelium and phloem. The promoters of SpsLAZY1a and SpsLAZY1b genes were non-tissue-specific，and their activities of the promoters were different. Collectively，this study provides a reference for analyzing the regulation mechanism of LAZY gene.