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Home > Archive>Volume 23, Issue 3, 2022 >738-745. DOI:10.13430/j.cnki.jpgr.20211021003
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Optimizing of Anther Culture System and Screening Wheat Genotypes with Higher Regeneration Ability
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Affiliation:

1.College of Agriculture,Yangtze University/Hubei Collaborative Innovation Center for Grain Industry,Hubei Jingzhou;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement

Fund Project:

Nuclear Energy Development Research Program of the State Administration of Science，Technology and Industry for National Defense“Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation”

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    Abstract:

    The high rate of albino plants is an issue in wheat anther culture system. In this study，carbon sources with different components were selected to optimize the anther culture system，followed by evaluating the culture ability of 22 wheat varieties （lines） samples harvested at two consecutive years. The anthers cultured in vitro with Sinopharm sucrose did not produce embryoids，whereas anthers succeeded in dedifferentiation into embryoids and further regenerate plantlets when using maltose from Sigma and Phytotech. No significant difference in the green plantlets per 100 anthers，but a significant difference in the albino plantlets per 100 anthers between the two kinds of maltose were observed. The albino plantlets per 100 anthers using Sigma maltose was significantly higher than that using Phytotech maltose. Moreover，the green/albino plantlets per 100 anthers was significantly different among 22 varieties （lines）. The genotypes showing high green plantlet regeneration ability were henong 6425 （33.97%），Luomai 28 （22.28%） and Zhengmai 136 （15.63%），and the genotypes showing high albino plantlet regeneration ability were Xiaoyan 22 （39.69%），Zhengmai 136 （33.99%）， Luomai 28 （42.17%） and Yunong 903 （28.59%），and the genotypes showing high plantlet regeneration ability were Xiaoyan 22 （46.69%），Zhengmai 136 （49.62%），Luomai 28 （64.45%），Henong 6425 （41.47%） and Yunong 903 （31.69%）. Through identifying the genotypes with high regeneration ability，this study provided the genotypes suitable for haploid breeding，gene mapping and genetic transformation in common wheat.

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History
  • Received:October 21,2021
  • Revised:February 08,2022
  • Adopted:February 09,2022
  • Online: May 10,2022
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